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Mirus Bio公司是世界上最早开发高效、低细胞毒性转染试剂的公司之一,同时也是目前该类试剂最主要的供应商之一。Mirus Bio的大部分转染试剂可以在含有血清的培养基中进行转染,省去了更换培养基的麻烦。TransIT-L1是Mirus产品的王牌,自1995年开发出来就申请了美国专利(US 5,744,335)。该项专利技术后来License给了Roche公司,以Fugene 6的品牌对外销售。TransIT-TKO & siQUEST转染试剂是常规siRNA转染试剂的王牌,与脂质体介导的转染试剂相比,该转染试剂可以高效转染siRNA,并且极大地降低了细胞的损伤程度。该试剂与siRNA混合转染细胞时,可以特异性剔除某些基因的表达,用于siRNA介导的基因沉默现象的研究。TransIT-Oligo转染试剂是即用型试剂,可以高效地将各种类型的寡聚核苷酸和寡聚脱氧核苷酸转染至受体细胞中。

 

此外,Mirus Bio还开发了专门针对特殊细胞的体外转染试剂,例如分别针对Jurkat,COS,3T3等细胞的转染试剂。Mirus Bio的科学家们新近开发了一种新的DNA转染试剂:TransITTM-2020 Transfection Reagent。TransITTM的性能优于FuGENETM、LipofectamineTM 2000和 LipofectamineTM 2000 CD;可在多种类型的细胞(包括难转染细胞)中实现高表达。

 

Mirus was founded on the principle that non-viral (i.e. plasmid DNA based) methods offer distinct advantages over viral methods of gene transfer. Our initial breakthrough in 1996 came with the development of a new type of transfection reagent that combined high efficiency delivery with low cellular toxicity: TransIT®-LT1 (Low Toxicity) Reagent remains the “gold standard” for broad-based gene delivery applications.

 

Mirus Bio公司主要产品:

Transfection with TransIT

Ingenio® Electroporation

Virus Production

Labeling with Label IT®

In Vivo Delivery

Accessory Products


品名货号
Human IgG1 Expression Control – 100 ugMIR 6250
Ingenio EZporator Electroporation SystemMIR 51000
Label IT Nucleic Acid Labeling Kit, Amine – sufficient for modifying 25 µg of nucleic acidMIR 3925
Label IT Nucleic Acid Labeling Kit, Amine – sufficient for modifying 100 µg of nucleic acidMIR 3900
TransIT-2020 Transfection Reagent – 0.4 mLMIR 5404
TransIT-2020 Transfection Reagent – 1.0 mLMIR 5400
TransIT-2020 Transfection Reagent – 10 X 1.0 mLMIR 5406
TransIT-2020 Transfection Reagent – 5 X 1.0 mLMIR 5405
TransIT-293  Transfection Reagent – 0.4 mLMIR 2704
TransIT-293 Transfection Reagent – 1.0 mLMIR 2700
TransIT-293 Transfection Reagent – 10 X 1.0 mLMIR 2706
TransIT-293 Transfection Reagent – 5 X 1.0 mLMIR 2705
TransIT-CHO  Transfection Kit – 10 X 1.0 mLMIR 2176
TransIT-CHO  Transfection Kit – 5 X 1.0 mLMIR 2175
TransIT-CHO Transfection Kit – 1.0 mLMIR 2170
TransIT-HeLaMONSTER Transfection Kit – 0.4 mLMIR 2904
TransIT-HeLaMONSTER Transfection Kit – 1.0 mLMIR 2900
TransIT-HeLaMONSTER Transfection Kit – 10 X 1.0 mLMIR 2906
TransIT-HeLaMONSTER Transfection Kit – 5 X 1.0 mLMIR 2905
TransIT-Insect Transfection Reagent – 0.4 mLMIR 6104
TransIT-Insect Transfection Reagent – 1.0 mLMIR 6100
TransIT-Insect Transfection Reagent – 10 X 1.0 mLMIR 6106
TransIT-Insect Transfection Reagent – 5 X 1.0 mLMIR 6105
TransIT-Jurkat Transfection Reagent – 0.4 mLMIR 2124
TransIT-Jurkat Transfection Reagent – 1.0 mLMIR 2120
TransIT-Jurkat Transfection Reagent – 10 X 1.0 mLMIR 2126
TransIT-Jurkat Transfection Reagent – 5 X 1.0 mLMIR 2125
TransIT-Keratinocyte Transfection Reagent – 0.4 mLMIR 2804
TransIT-Keratinocyte Transfection Reagent – 1.0 mLMIR 2800
TransIT-Keratinocyte Transfection Reagent – 10 X 1.0 mLMIR 2806
TransIT-Keratinocyte Transfection Reagent – 5 X 1.0 mLMIR 2805
TransIT-LT1 Transfection Reagent – 0.4 mLMIR 2304
TransIT-LT1 Transfection Reagent – 5 X 1.0 mLMIR 2305
TransIT-mRNA Transfection Kit – 0.4 mLMIR 2225
TransIT-mRNA Transfection Kit – 1.0 mLMIR 2250
TransIT-mRNA Transfection Kit – 10 X 1.0 mLMIR 2256
TransIT-mRNA Transfection Kit – 5 X 1.0 mLMIR 2255
TransIT-Oligo Transfection Reagent – 0.4 mLMIR 2164
TransIT-Oligo Transfection Reagent – 1.0 mLMIR 2160
TransIT-Oligo Transfection Reagent – 10 X 1.0 mLMIR 2166
TransIT-Oligo Transfection Reagent – 5 X 1.0 mLMIR 2165
TransIT-PRO Transfection Kit – 1.0 mL (with boost)MIR 5700
TransIT-PRO Transfection Kit – 10 mL (with boost)MIR 5760
TransIT-PRO Transfection Reagent – 1 mL (without boost)MIR 5740
TransIT-PRO Transfection Reagent – 10 mL (without boost)MIR 5750
TransIT-siQUEST Transfection Reagent – 0.4 mLMIR 2114
TransIT-siQUEST Transfection Reagent – 1.5 mLMIR 2110
TransIT-siQUEST Transfection Reagent – 10 X 1.5 mLMIR 2116
TransIT-siQUEST Transfection Reagent – 5 X 1.5 mLMIR 2115
TransIT-TKO Transfection Reagent – 0.4 mLMIR 2154
TransIT-TKO Transfection Reagent – 1.5 mLMIR 2150
TransIT-TKO Transfection Reagent – 10 X 1.5 mLMIR 2156
TransIT-TKO Transfection Reagent – 5 X 1.5 mLMIR 2155
TransIT-X2 Dynamic Delivery System – 0.3 mLMIR 6003
TransIT-X2 Dynamic Delivery System – 0.75 mLMIR 6004
TransIT-X2 Dynamic Delivery System – 1.5mLMIR 6000
TransIT-X2 Dynamic Delivery System – 10 X 1.5 mLMIR 6006
TransIT-X2 Dynamic Delivery System – 5 X 1.5 mLMIR 6005
Ingenio Electroporation Accessory – Cell Droppers (50 pk)MIR 50125
Ingenio Electroporation Solution Only – 25 rxns (6.25 mL)MIR 50111
Label IT Nucleic Acid Labeling Kit, Biotin – labels 100 µg nucleic acidMIR 3400
Label IT Plasmid Delivery Control, Cy3 – 10 µgMIR 7904
Label IT RNAi Delivery Control, Cy3 – 10 µg (0.75 nmol)MIR 7900
TransduceIT Transduction Reagent – 1 mLMIR 6620
TransIT-BrCa Reagent Transfection Reagent – 0.4 mLMIR 5504
Ingenio Electroporation Accessory – 0.4 cm Cuvettes (50 pk)MIR 50123
Ingenio Electroporation Solution Only – 50 rxns (12.5 mL)MIR 50114
Label IT Nucleic Acid Labeling Kit, Biotin – labels 25 µg nucleic acidMIR 3425
Label IT Plasmid Delivery Control, Cy3 – 100 µgMIR 7905
Label IT RNAi Delivery Control, Cy3 – 100 µg (7.5 nmol)MIR 7901
Label IT siRNA Tracker Intracellular Localization Kit, CX-Rhodamine – labels 50 µg siRNAMIR 7214
Label IT Tracker Intracellular Nucleic Acid Localization Kit, CX-Rhodamine – labels 50-200 µg plasmid DNAMIR 7022
Lentivirus Packaging Mix – 34 rxnMIR 6640
TransIT-BrCa Reagent Transfection Reagent – 1.0 mLMIR 5500
TransIT-LT1 Transfection Reagent – 1.0 mLMIR 2300
Ingenio Electroporation Accessory – 0.2 cm Cuvettes (50 pk)MIR 50121
Ingenio Electroporation Solution Only – 100 rxns (25 mL)MIR 50117
Label IT Nucleic Acid Labeling Kit, CX-Rhodamine – labels 100 µg nucleic acidMIR 3100
Label IT Plasmid Delivery Control, Fluorescein – 10 µgMIR 7906
Label IT RNAi Delivery Control, Fluorescein – 10 µg (0.75 nmol)MIR 7902
Label IT siRNA Tracker Intracellular Localization Kit, Cy3 – labels 50 µg siRNAMIR 7212
Label IT Tracker Intracellular Nucleic Acid Localization Kit, Cy3 – labels 50-200 µg plasmid DNAMIR 7020
Lentivirus Packaging Mix – 5 rxnMIR 6630
TransIT-BrCa Transfection Reagent – 5 x 1.0 mLMIR 5505
Ingenio Electroporation Kit, for Amaxa – 25 rxns (0.2 cm Cuvettes/Droppers)MIR 50112
Label IT Nucleic Acid Labeling Kit, CX-Rhodamine – labels 25 µg nucleic acidMIR 3125
Label IT Plasmid Delivery Control, Fluorescein – 100 µgMIR 7907
Label IT RNAi Delivery Control, Fluorescein – 100 µg (7.5 nmol)MIR 7903
Label IT siRNA Tracker Intracellular Localization Kit, Cy5 – labels 50 µg siRNAMIR 7213
Label IT Tracker Intracellular Nucleic Acid Localization Kit, Cy5 – labels 50-200 µg plasmid DNAMIR 7021
TransIT Lentivirus System – 34 rxnMIR 6655
TransIT-BrCa Transfection Reagent – 10 x 1.0 mLMIR 5506
TransIT-LT1 Transfection Reagent – 10 X 1.0 mLMIR 2306
Ingenio Electroporation Kit, for Amaxa – 50 rxns (0.2 cm Cuvettes/Droppers)MIR 50115
Label IT Nucleic Acid Labeling Kit, Cy3 – labels 100 µg nucleic acidMIR 3600
Label IT siRNA Tracker Intracellular Localization Kit, Fluorescein – labels 50 µg siRNAMIR 7216
Label IT Tracker Intracellular Nucleic Acid Localization Kit, Fluorescein – labels 50-200 µg plasmid DNAMIR 7025
TransIT Lentivirus System – 5 rxnMIR 6650
Ingenio Electroporation Kit, for Amaxa – 100 rxns (0.2 cm Cuvette/Droppers)MIR 50118
Label IT Nucleic Acid Labeling Kit, Cy3 – labels 25 µg nucleic acidMIR 3625
TransIT-Lenti Transfection Reagent – 10 x 1.5 mLMIR 6606
Ingenio Electroporation Kit, for other Electroporators – 25 rxns, (0.4 cm Cuvettes/Droppers)MIR 50113
Label IT Nucleic Acid Labeling Kit, Cy5 – labels 100 µg nucleic acidMIR 3700
TransIT-Lenti Transfection Reagent – 1.5 mLMIR 6600
Ingenio Electroporation Kit, for other Electroporators – 50 rxns (0.4 cm Cuvettes/Droppers)MIR 50116
Label IT Nucleic Acid Labeling Kit, Cy5 – labels 25 µg nucleic acidMIR 3725
TransIT-Lenti Transfection Reagent – 0.75 mLMIR 6604
Ingenio Electroporation Kit, for other Electroporators – 100 rxns (0.4 cm Cuvette/Droppers)MIR 50119
TransIT-Lenti Transfection Reagent – 5 x 1.5 mLMIR 6605
TransIT-Lenti Transfection Reagent – 0.3 mLMIR 6603
Label IT Nucleic Acid Labeling Kit, DNP – labels 100 µg nucleic acidMIR 3800
Label IT Nucleic Acid Labeling Kit, DNP – labels 25 µg nucleic acidMIR 3825
Label IT Nucleic Acid Labeling Kit, Fluorescein – labels 100 µg nucleic acidMIR 3200
Label IT Nucleic Acid Labeling Kit, Fluorescein – labels 25 µg nucleic acidMIR 3225
Label IT Nucleic Acid Labeling Kit, MFP488 – labels 100 µg nucleic acidMIR 7100
Label IT Nucleic Acid Labeling Kit, MFP488 – labels 25 µg nucleic acidMIR 7125
TransIT-EE Delivery Solution – 40 InjectionsMIR 5340
TransIT-QR Delivery Solution – 40 InjectionsMIR 5240
pLIVE Vector – 20 ugMIR 5420
pLIVE Vector/LacZ – 20 ug of each vectorMIR 5520
pLIVE Vector/SEAP Control Vector Kit – 20 ug of each vectorMIR 5620
pLIVE Vector Complete System – 20 ug of each vectorMIR 5320
RevIT AAV Enhancer – 1.5 mL (for 1 L of cell culture)MIR 8000
TransIT-VirusGEN GMP Transfection Reagent – 150 mLMIR 6845-GMP
VirusGEN AAV Transfection Kit – 150mLMIR 6755
VirusGEN AAV Transfection Kit, 1 Kit – for 10 L of cell cultureMIR 6745
RevIT AAV Enhancer – 10 x 1.5 mL (for 10 L of cell culture)MIR 8006
RevIT AAV Enhancer – 75 mL (for 50 L of cell culture)MIR 8080
VirusGEN GMP LV Transfection KitMIR 6825-GMP
VirusGEN LV Transfection KitMIR 6760
VirusGEN AAV Kit + RevIT for 10L CultureMIR 8008
VirusGEN GMP AAV Transfection KitMIR 6815-GMP
VirusGEN AAV Transfection Kit – 1 Kit for 1 L of cell cultureMIR 6750
VirusGEN AAV Kit + RevIT for 1L CultureMIR 8007
TransIT-VirusGEN Transfection Reagent – 150 mLMIR 6740
TransIT-VirusGEN Transfection Reagent – 30 mLMIR 6720
TransIT-VirusGEN Transfection Reagent – 10 x 1.5 mLMIR 6706
TransIT-VirusGEN Transfection Reagent – 5 x 1.5 mLMIR 6705


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艾美捷科技有限公司

全国服务热线:400-6800-868

邮箱:sales@amyjet.com

网站:www.amyjet.com


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Protide-logo-1-600x314.png

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艾美捷科技代理Protide Pharmaceuticals品牌全系列产品


Protide Pharmaceuticals, Inc.是一家专注于生物制药生产上游工艺的创新型公司。Protide Pharmaceuticals公司的核心产品为血清替代物,其核心价值在于提供高性能、化学成分明确、无动物来源的血清替代物和定制化培养基解决方案,帮助客户大幅减少生物制品生产过程中对胎牛血清或其他动物血清的依赖。,从而提高生物制品生产的效率、一致性、安全性和合规性。

除了血清替代物,Protide Pharmaceuticals公司也提供完整的、优化的无血清培养基配方,专门设计用于支持各种哺乳动物细胞系(如CHO, HEK293, Vero, MRC-5等)在生物反应器中进行高密度培养和高产率生产目标生物分子(如抗体、病毒载体、重组蛋白)。其产品应用于再生医学、肿瘤生物学、细胞生物能量学、肿瘤免疫学、转化医学、表观遗传学,胰岛细胞移植等领域。


ProtidePharmaceuticals页面.png

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Protide Pharmaceuticals产品线

血清替代品/生物制品生产培养基

Protide Pharmaceuticals的血清替代品通过提供优质、生物一致且功能性的环境,显著改善细胞的生理状态。所有血清替代品均配制成 50 倍浓缩液,这大大降低了成本,并改善了储存和供应链活动。Protide血清替代品不含:酚红、生长因子、类固醇激素、糖皮质激素、细胞粘附分子、生长因子、类固醇激素等物质,可以在其缺失或存在的情况下进行有效研究。细胞分泌的蛋白质可以在不受污染性血清成分干扰的情况下实现高度纯化。


品名货号-规格产品描述
TCH™3001-40ml;3002-100ml;3005-100ml本血清替代品主要用于人类细胞培养及细胞分泌蛋白的生产。TCH™ 在培养其他哺乳动物细胞时同样有效,且不含动物蛋白。其成分仅包含经热处理的人源蛋白(即人血清白蛋白、人转铁蛋白、重组人胰岛素)。
VaxMax™6004-100ml;6005-500ml;6006-1L100倍浓缩液是一种高性价比产品,可显著降低总体血清需求量。在MDBK、MDCK、Vero、CRFK及猪睾丸细胞培养中,使用VaxMax™已展现出显著的产量提升。兽用疫苗生产商已采用VaxMax™来增强生产,同时降低总体成本。VaxMax™通常与0.5%至1% 的血清配合使用。
HemaPro™3300-40ml;3303-100ml本产品为无血清添加剂,专为人祖细胞培养而设计。可有效用于癌症干细胞、诱导多能干细胞和间充质干细胞 的研究。不含:促红细胞生成素;重组生长因子。
TM-235™2001-40ml;2002-100ml;2005-500ml本血清替代品具有与TCM™类似的特性,但额外添加了适用于需要更高血清浓度(通常超过10%)的细胞类型的成分。TM-235™是一种成分明确且多用途的替代品。
TCM™1001-40ml;1002-100ml;1005-500ml本产品是一种通用型血清替代品,适用于多种物种来源的广泛细胞类型。TCM™是一种成分明确且多用途的血清替代品,可有效用于研究生长因子的影响以及蛋白质的生产。


● 低温保护剂


品名货号-规格产品描述
Stemsol™ USP (DMSO)PP1250-5ml;PP1300-10ml;PP1350-50ml;PP1400-100ml本产品为干细胞冻存溶液,适用于来源于脐带血、外周血、骨髓及其他生物组织和细胞的干细胞。
pZerve™5700-60ml;5720-20mlpZerve™ 是一款独特的冻存溶液,不含 DMSO、胎牛血清或动物蛋白。本品为即用型,无需任何稀释或进一步处理。适用细胞类型包括:肥大细胞、树突细胞、间充质细胞、人转移性前列腺腺癌细胞、人结肠腺癌细胞,人转化胚肾细胞。
Cellvation™5001-60mlCellvation™ 是一款独特的冻存溶液,不含 DMSO 或胎牛血清。本品使用便捷,并能提供最佳的细胞复苏效果。在冷冻程序启动前,细胞可置于 Cellvation™ 中较长时间。本品为即用型,适用于无血清培养基或含血清培养基培养的细胞。
MiCord™ 55% DMSO USP 5% Dextran USP1505-5ml;1500-7ml;;1700-10ml;1550-50ml;1600-100ml本产品为干细胞冻存溶液,适用于来源于脐带血、外周血、骨髓及其他生物组织和细胞的干细胞。
2-8CELLsius™ + DMSO,5% DMSOPP338DM5-10ml;PP338DM5-100ml;PP338DM5-100B当与DMSO配合使用时,2-8CELLsius™可用作冻存液。2-8CELLsius™ + DMSO冻存液是一种细胞保护性、无蛋白溶液,含有生物稳定剂,可支持冻融后细胞的高存活率。其通过针对超低温下细胞生理学的创新方法,实现最佳的细胞复苏效果。不含:任何生长因子,激素以及抗生素。
2-8CELLsius™ + DMSO,10% DMSOPP338DM10-10ml;PP338DM10-100ml;PP338DM10-100B;PP338DM10-1000B
2-8CELLsius™ + DMSO,20% DMSOPP338DM20-100B;PP338DM20-1000B


细胞处理


品名货号-规格产品描述
2-8CELLsius™PP338-100ml;PP338-500ml;PP338-1000;PP338-1000B即用型、平衡、细胞保护性、无蛋白溶液,含生物稳定剂,适用于:短期储存、再生医学、干细胞处理以及生物材料运输。
Dextran 40 in 0.9% NaClPP1850-50ml;PP1840-1000ml;PP1880-250ml;PP1900-500ml-
Dulbecco’s Phosphate Buffered Saline (10X) PBSPBS210-500ml;PBS211-1000ml无钙镁储存条件:15-30°C;包装于PETG瓶;稳定性:至少6个月;无菌过滤
Dulbecco’s Phosphate Buffered Saline (1X) PBSPBS200-500ml;PBS201-1000ml无钙镁储存条件:15-30°C;包装于PETG瓶;稳定性:至少6个月;无菌过滤
Hank’s Balanced Salt Solution (1X) HBSSHS240-500ml;HS241-1000ml无钙镁配方:储存温度 4-8°C;无酚红配方;稳定性:至少6个月;含0.35克/升 碳酸氢钠;含1000毫克/升 D-葡萄糖;包装于 PETG 瓶,无菌过滤。cGMP 规范生产。


● CIT 胰岛细胞溶液


品名货号-规格
Cold Storage/Purification Stock Solution111098-1L
Phase I Solution060899-1L


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